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Catalog Number | orb80037 |
---|---|
Category | Proteins |
Description | Interferon-alpha 1b Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton.The Interferon-alpha 1b gene was obtained from human leukocytes.The IFN-a 1b is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | IFN 1b |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IFN alpha 1b in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu. |
Biological Activity | The specific activity as determined in viral resistance assay using bovine kidney MDBK cells was found to be x 100,000,000 IU/ mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from (1 mg/ml) solution in containing 20 mg HSA and 7.5 mg sodium chloride. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Interferon-alpha 1b protein
Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Greater than 90% as determined by SDS-PAGE. | |
35.4 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
35.4 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
35.4 kDa | |
E.coli |
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