Human IL5Ra ELISA Kit

• Catalog Number: orb777828
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 Receptor Alpha(IL5Ra).
• Tested applications: ELISA
• Reactivity: Human
• Range: 78.13-5000 pg/mL
• Concentration: 5000 pg/mL
• UniProt ID: Q01344
• Sensitivity: 25.1 pg/mL
• Protein Length: 3.5h
• Alternative names: CDw125 ELISA Kit, CD125 ELISA Kit, IL5R-A ELISA Ki
• Note: For research use only
• Application notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL5Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL5Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL5Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL5Ra in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma and other biological fluids.
• Expiration Date: Please enquire.