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Human IL1RA protein
Catalog Number: orb80054
| Catalog Number | orb80054 |
|---|---|
| Category | Proteins |
| Description | Recombinant human IL1RA protein |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | IL1RA |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin-1ra in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | Lyophilized protein with no additives. |
| Alternative names | DIRA protein, ICIL 1RA protein, ICIL-1RA protein, Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.89 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-1 ra as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL1RA protein
- Item 1 of 3
Anti-IL1RA/IL1RN Antibody [orb402389]
ELISA, FC, IHC, WB
Human
Rabbit
Polyclonal
Unconjugated
10 μg, 100 μg - Item 1 of 1
Human IL1RA protein (Active) [orb358967]
> 96% as determined by SDS-PAGE and HPLC.
17.1 kDa
E.Coli
100 μg, 500 μg - Item 1 of 1
Human IL1RA Protein, hFc Tag [orb1173740]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 62.3 kDa after removal of the signal peptide.The apparent molecular mass of IL1RA-hFc is approximately 55-70 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 1
Human IL1RA protein [orb594814]
Greater than 95% as determined by SDS-PAGE.
17.26 kDa
E.coli
10 μg, 50 μg, 500 μg, 1 mg - Item 1 of 1
Human IL1RA Protein, His Tag [orb2321377]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 37.5 kDa after removal of the signal peptide. The apparent molecular mass of IL1RA-His is approximately 35-70 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
