Human IL12 protein

• Catalog Number: orb80072
• Category: Proteins
• Description: IL12 Human Recombinant produced in CHO is glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: IL12
• Solubility (25°C): It is recommended to reconstitute the lyophilized Interleukin-12 in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.
• Biological Activity: Measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/mL.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: Measured by its ability to stimulate the proliferation of PHA-activated human lymphoblasts. The ED50 for this effect is 0.3-0.5 ng/mL.. Lyophilized from 0.2 um filtered solution (0.5mg/ml) in PBS, pH 7.4.
• Alternative names: NKSF protein, CTL maturation factor (TCMF) protein
• Note: For research use only
• Expiration Date: 6 months from date of receipt.