Human IL-8 / CXCL8 Protein

• Catalog Number: orb612149
• Category: Proteins
• Description: Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 10.3 kDa
• Target: IL-8
• Source: Human IL-8, His Tag (orb612149) is expressed from human 293 cells (HEK293). It contains AA Ser 28 - Ser 99 (Accession # P10145-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-8 Protein, His Tag, premium grade at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
• Expression Region: Ser 28 - Ser 99
• Endotoxins: 0.1 EU per μg
• NCBI: NP_000575.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: CXCL8,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 10.3 kDa. The protein migrates as 13 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-8 Protein, His Tag, premium grade (Cat. No. orb612149) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL-8 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).