Human IL-6 ELISA Kit

• Catalog Number: orb1173350
• Category: Assays and Kits
• Description: Human IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-6.
• Tested applications: ELISA
• Reactivity: Human
• Tag: IL6
• Entrez: 3569
• UniProt ID: P05231
• Storage: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
• Alternative names: IL 6, IL6, BSF2, HGF, HSF, IFNB2, IL-6, B cell sti
• Note: For research use only
• Application notes: Human IL-6 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-6 in samples. An antibody specific for IL-6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-6 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Sample Types: Cell culture supernatants#Serum#Plasma#Other biological fluids
• Expiration Date: Please enquire.