Human IL-35 Protein

• Catalog Number: orb1184774
• Category: Proteins
• Description: Interleukin 35 (IL-35) is a recently discovered anti-inflammatory cytokine from the IL-12 family. Member of IL-12 family - IL-35 is produced by wide range of regulatory lymphocytes and plays a role in immune suppression. IL-35 can block the development of Th1 and Th17 cells by limiting early T cell proliferation. The heterodimeric cytokine of IL-35 involves two subunits of p35 and Ebi3 which are also seen in IL-12 and IL-27. IL-35 is recognized as a defi nite immunosuppressor with a huge potent of suppression.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 73.3 kDa
• Target: IL-35
• Source: Human IL-35, Fc Tag (orb1184774) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha) (Accession # Q14213-1 (IL-27 beta) & P29459-1 (IL-12 alpha)).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-35 R, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag with a linear range of 0.002-0.625 μg/mL (QC tested).
• Expression Region: Arg 21 - Lys 229 (IL-27 beta) & Arg 23 - Ser 219 (IL-12 alpha)
• Endotoxins: 1.0 EU per μg
• NCBI: NP_005746.2, NP_000873.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: EBI3,IL35,IL-35EBI3,IL35p35,Interleukin-35,IL-35
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.3 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-35 R, His Tag (Cat. No. orb1710809) at 5 μg/mL (100 μL/well) can bind Human IL-35, Fc Tag (Cat. No. orb1184774) with a linear range of 0.002-0.625 μg/mL (QC tested).