Human IL-3 R Beta / CD131 Protein

• Catalog Number: orb1176553
• Category: Proteins
• Description: CD131- the common β Subunit, is a member of the type 1 cytokine receptor family. There are three major classes of heteroreceptor complexes, where the receptor complex utilises a common shared receptor subunit as a signal-transducing chain along with a cytokine specific subunit. These three classes include those which use gp130, those which use γc and those which use the common β subunit, βc(CD131). CD131 is shared by three of the “four-helical bundle” family of cytokines; IL-3, IL-5 and GM-CSF, and forms a receptor complex along with a cytokine specific α chain for each of these cytokines.Moreover, even though each of these cytokines have their own specific α subunit, the co-utilisation of CD131 suggests a common intra-cellular process of both unique and/or overlapping activities on expressing cells.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 75.1 kDa
• Target: CD131
• Source: Human CD131, Fc Tag (orb1176553) is expressed from human 293 cells (HEK293). It contains AA Trp 17 - Trp 443 (Accession # P32927-1).
• Expression System: HEK293
• Biological Activity: Loaded Human CD131, Fc Tag on Protein A Biosensor, can bind Human GM-CSF, premium grade with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: Trp 17 - Trp 443
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000386.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Alternative names: IL-3 R Beta,CD131,Common beta Chain
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 75.1 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Loaded Human CD131, Fc Tag (Cat. No. orb1176553) on Protein A Biosensor, can bind Human GM-CSF, premium grade (Cat. No. orb257510) with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).