Human IL-2 R beta / CD122 Protein

• Catalog Number: orb257617
• Category: Proteins
• Description: Interleukin-2 receptor (IL-2R) is a heterotrimeric protein expressed on the surface of certain immune cells, such as lymphocytes, that binds and responds to a cytokine called IL-2. The IL-2R is made up of 3 subunits - α (CD25), β (CD122) and γ (CD132) - non-covalently associating. The α and β chains are involved in binding IL-2, while signal transduction following cytokine interaction is carried out by the γ-chain, along with the β subunit. CD122 is also known as IL2R beta, is a member of the type I cytokine receptor family. CD122 is the receptor for interleukin-2 and is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 51.0 kDa
• Target: IL-2 R beta
• Source: Human IL-2 R beta, Fc Tag (orb257617) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (Accession # NP_000869.1).
• Expression System: HEK293
• Biological Activity: Human IL-2 R beta, Fc Tag captured on CM5 chip via anti-human IgG Fc antibody can bind IL-2, Tag Free with an affinity constant of 0.473 μM as determined in a SPR assay (Biacore T200) (QC tested).
• Expression Region: Ala 27 - Asp 239
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000869.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
• Alternative names: IL2RB,RP5-1170K4.6,CD122,P70-75
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.0 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-2 R beta protein

Human IL-2 R beta, Fc Tag (Cat. No. orb257617) captured on CM5 chip via anti-human IgG Fc antibody can bind IL-2, Tag Free with an affinity constant of 0.473 μM as determined in a SPR assay (Biacore T200) (QC tested).