Human IL-2 (N108D, C145A) & TSG-6 Protein, His Tag (MALS verified)

• Catalog Number: orb1710806
• Category: Proteins
• Description: Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 28.3 kDa
• Target: IL-2 & TSG-6
• Source: Human IL-2 (N108D, C145A) & TSG-6, His Tag (orb1710806) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 & Gly 36 - Asn 129 (Accession # P60568-1 (N108D, C145A) & P98066-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-2 R alpha Protein, Fc Tag at 2 μg/mL (100 μL/well) can bind Human IL-2 (N108D, C145A) & TSG-6, His Tag with a linear range of 5-40 ng/mL (QC tested).
• Expression Region: Ala 21 - Thr 153 & Gly 36 - Asn 129
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 100 mM NaAc,25 mM Arginine,pH5.5
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.3 kDa. The protein migrates as 32-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human IL-2 R alpha Protein, Fc Tag (Cat. No. orb257608) at 2 μg/mL (100 μL/well) can bind Human IL-2 (N108D, C145A) & TSG-6, His Tag (Cat. No. orb1710806) with a linear range of 5-40 ng/mL (QC tested).