Human IL-18 R beta / IL-1 R7 Protein

• Catalog Number: orb1101190
• Category: Proteins
• Description: IL-18 R beta (IL-1R7), also known as IL18RAP (Interleukin 18 Receptor Accessory Protein), is a member of the IL-1 family. Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 64.9 kDa
• Target: IL-18 R beta
• Source: Human IL-18 R beta Protein, Fc Tag (orb1101190) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Arg 356 (Accession # O95256-1).
• Expression System: HEK293
• Biological Activity: Biotinylated Human IL-18 R1, His, captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free (Premixed with Human IL-18 R beta, Fc Tag) with an affinity constant of 5.87 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Phe 20 - Arg 356
• Endotoxins: 1.0 EU per μg
• NCBI: NP_003844.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Alternative names: IL-18 R beta,IL-1 R7,CD218b,CDw218b,IL-1 R7,IL-18
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Biotinylated Human IL-18 R1, His, Avitag (Cat. No. orb1184767) captured on Biotin CAP-Series S Sensor Chip can bind Human IL-18, Tag Free (Premixed with Human IL-18 R beta, Fc Tag, Cat. No. orb1101190) with an affinity constant of 5.87 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).