Human IL-12B / NKSF2 / p40 Protein

• Catalog Number: orb257708
• Category: Proteins
• Description: Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. Interleukin-12 subunit beta (IL12B) also known as NKSF2, CLMF2 and P40. Interleukin-12 subunit beta has been shown to interact with IL23. A large excess of monomeric IL12B is also secreted by the cells producing IL12, and exhibits no demonstrable biological activity. Overexpression of IL12B gene has been shown to be associated with the pathogenesis of multiple sclerosis. In addition, studies have revealed that the promoter polymorphism of this gene is implicated in the severity of atopic and non-atopic asthma in children.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 61.3 kDa
• Target: IL-12B
• Source: Human IL-12B, Fc Tag (orb257708) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Ser 328 (Accession # AAH67499).
• Expression System: HEK293
• Biological Activity: Immobilized Human IL-12 R beta 1, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag with a linear range of 0.039-0.625 μg/mL (QC tested).
• Expression Region: Ile 23 - Ser 328
• Endotoxins: 1.0 EU per μg
• NCBI: AAH67499
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL12B,CLMF,CLMF2,NKSF,NKSF2,p40
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 61.3 kDa. The protein migrates as 66-76 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-12B protein

Immobilized Human IL-12 R beta 1, His Tag (Cat. No. orb570283) at 2 μg/mL (100 μL/well) can bind Human IL-12B, Fc Tag (Cat. No. orb257708) with a linear range of 0.039-0.625 μg/mL (QC tested).