Human IL-10 R alpha / CD210 Protein

• Catalog Number: orb1496310
• Category: Proteins
• Description: Interleukin-10 receptor subunit alpha (IL-10 R alpha ) is a cell surface receptor for the cytokine IL-10 that participates in IL-10 mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well.In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively.These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 50.8 kDa
• Target: IL-10 R alpha
• Source: Human IL-10 R alpha Protein, Fc Tag (orb1496310) is expressed from human 293 cells (HEK293). It contains AA His 22 - Asn 235 (Accession # Q13651).
• Expression System: HEK293
• Biological Activity: Loaded Human IL-10 R alpha / CD210, Fc Tag on Protein A Biosensor, can bind Human IL-10, His Tag with an affinity constant of 1.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Expression Region: His 22 - Asn 235
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Interleukin-10 receptor subunit alpha,IL-10 recept
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.8 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Loaded Human IL-10 R alpha / CD210, Fc Tag (Cat. No. orb1496310) on Protein A Biosensor, can bind Human IL-10, His Tag (Cat. No. orb334903) with an affinity constant of 1.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).