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Catalog Number | orb257592 |
---|---|
Category | Proteins |
Description | Interleukin-1 receptor type 2 (IL1R2) is also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 63.9 kDa |
Target | IL-1 RII |
Source | Human IL-1 RII, Fc Tag (orb257592) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1). |
Expression System | HEK293 |
Biological Activity | Human IL-1 RII, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested). |
Expression Region | Phe 14 - Glu 343 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_004624.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, pH7.5 |
Alternative names | CD121b,IL1RB,IL1R2,CDw121b Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 63.9 kDa. The protein migrates as 70-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-1 RII protein
Line graph illustrates about the sensitivity range of Human IL-1 RII protein using different concentrations
Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. orb257615) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Unconjugated | |
98% | |
38.9 kDa | |
Human IL-1 RII, His Tag (orb257591) is expressed from human 293 cells (HEK293). It contains AA Phe 14 - Glu 343 (Accession # NP_004624.1). |
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