Human IL-1 beta / IL-1F2 Protein

• Catalog Number: orb257615
• Category: Proteins
• Description: Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 17.5 kDa
• Target: IL-1 beta
• Source: Human IL-1 beta, premium grade (orb257615) is expressed from E. coli cells. It contains AA Ala 117 - Ser 269 (Accession # P01584-1).
• Expression System: E. coli
• Biological Activity: Human IL-1 RII, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ala 117 - Ser 269
• Endotoxins: 0.02 EU per μg
• NCBI: NP_000567.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IL1B,IL-1BETA,IL1F2,IL-1β
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 17.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-1 beta protein

Human IL-1 RII, Fc Tag (Cat. No. orb257592) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-1 beta, premium grade (Cat. No. orb257615) with an affinity constant of 0.622 nM as determined in a SPR assay (Biacore T200) (Routinely tested).