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Catalog Number | orb867311 |
---|---|
Category | Proteins |
Description | Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA,IL1F1,and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum.The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes. |
Reactivity | Human |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 19.9 kDa |
Target | IL-1 alpha |
Source | Human IL-1 alpha, His Tag (orb867311) is expressed from human 293 cells (HEK293). It contains AA Ser 113 - Ala 271 (Accession # P01583-1). |
Expression System | HEK293 |
Biological Activity | Immobilized Human IL-1 RI Protein, Fc Tag at 10 μg/mL (100 μL/well) can bind Human IL-1 alpha, His Tag with a linear range of 0.3-2.5 μg/mL (QC tested). |
Expression Region | Ser 113 - Ala 271 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000566.3 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL1A,IL-1 ALPHA,IL1F1,IL1α Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.9 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Human IL-1 RI Protein, Fc Tag at 10 μg/mL (100 μL/well) can bind Human IL-1 alpha, His Tag (Cat. No. orb867311) with a linear range of 0.3-2.5 μg/mL (QC tested).
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