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Catalog Number | orb347297 |
---|---|
Category | Antibodies |
Description | Human IgM (Fc5u) antibody (Peroxidase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Human IgM Fc5µ fragment |
Concentration | 1.5 mg/mL |
Dilution range | ELISA: 1:15,000 - 1:60,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes. |
Conjugation | HRP |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Alternative names | Rabbit Anti-Human IgM (Fc5u) Antibody Peroxidase C Read more... |
Note | For research use only |
Application notes | Anti-Human IgM (Fc5µ) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by enzyme digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Human IgM, anti-Human IgM Fc5µ and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2. | |
Human |
ELISA, IHC, WB | |
Human | |
Goat | |
Polyclonal | |
HRP |
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