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    Human IgM (Fc5u) antibody (FITC)

    Human IgM (Fc5u) antibody (FITC)

    Catalog Number: orb347289

    DispatchUsually dispatched within 5-10 working days
    $ 352.00
    Catalog Numberorb347289
    CategoryAntibodies
    DescriptionHuman IgM (Fc5u) antibody (FITC)
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsDOT, FC, FLISA, IF
    ReactivityHuman
    IsotypeIgG
    ImmunogenHuman IgM Fc5µ fragment
    Concentration1.5 mg/mL
    Dilution rangeFLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
    ConjugationFITC
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesRabbit Anti-Human IgM (Fc5u) Antibody fluorescein
    Read more...
    NoteFor research use only
    Application notesAnti-Human IgM Fc5µ Fluorescein antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Expiration Date12 months from date of receipt.
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      This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by trypsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Human IgM Fc5µ and anti-Human Serum. No reaction was observed against anti-Human IgG F(ab’)2 or anti-Trypsin.

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      1 mg
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