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Catalog Number | orb347305 |
---|---|
Category | Antibodies |
Description | Human IgM (Fc5u) antibody (Alkaline Phosphatase) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Human IgM Fc5µ fragment |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes. |
Conjugation | AP |
Storage | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti-Human IgM (Fc5u) Antibody alkaline pho Read more... |
Note | For research use only |
Application notes | Anti-Human IgM Fc5µ Alkaline Phosphatase conjugate has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC, WB | |
Human | |
Goat | |
Polyclonal | |
AP |
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