Human IgG Fc Antibody Peroxidase Conjugated

• Catalog Number: orb347223
• Category: Antibodies
• Description: Human IgG F(c) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: Anti-HUMAN IgG F(c) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab).
• Immunogen: Human IgG F(c) fragment
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:60,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application notes: Anti-Human IgG F(c) Peroxidase Conjugated has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:6,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Biological Activity: 0.5
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat anti-Human IgG F(c) peroxidase Conjugated Ant
• Note: For research use only