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Description
Research Area
Protein Biochemistry
Images & Validation
−| Tested Applications | ELISA, FC, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human |
|---|---|
| Biological Origin | Human |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purity | IgG was prepared from normal human serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human IgG and anti-Human Serum. |
Storage & Handling
−| Storage | Store Human IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 10.846 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Human IgG whole molecule, Human Immunoglobulin G
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis



































































