Human IGFBP-3 Protein

• Catalog Number: orb1146966
• Category: Proteins
• Description: Insulin-like growth factor-binding protein 3 is also known as IGFBP3, is a protein that, in humans, is encoded by the IGFBP3 gene. IGFBP3 forms a ternary complex of about 140 ~150 kDa with IGF1 or IGF2 and a glycoprotein insulin-like growth factor acid-labile subunit (ALS), thus alter the interaction of IGFs with their cell surface receptors. IGFBP3 exerts either proapoptotic or growth stimulatory effects depending upon the cellular context. Studies have shown that IGFBP3 can leads to the induction of apoptosis dependent or independent of the IGF-IGF receptor axis, accordingly acts as a negative regulator of tumorigenesis and progressing in certain carcinomas. The highest expression level is found in the nonparanchymal cells of the liver. Expression levels are also higher during extrauterine life and peak during puberty.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 30.6 kDa
• Target: IGFBP-3
• Source: Human IGFBP-3, His Tag (orb1146966) is expressed from human 293 cells (HEK293). It contains AA Gly 28 - Lys 291 (Accession # P17936-1).
• Expression System: HEK293
• Biological Activity: Immobilized Biotinylated Human IGF-II, ,Fc Tag at 2 μg/mL (100 μL/well) on streptavidin precoated (1 μg/well) plate can bind Human IGFBP-3, His Tag with a linear range of 0.01-0.313 μg/mL (QC tested).
• Expression Region: Gly 28 - Lys 291
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000589.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGFBP3,BP-53,IBP3
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.6 kDa. The protein migrates as 40-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Biotinylated Human IGF-II, Avitag, Fc Tag at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. orb668907) precoated (1 μg/well) plate can bind Human IGFBP-3, His Tag (Cat. No. orb1146966) with a linear range of 0.01-0.313 μg/mL (QC tested).