Human IFN-gamma / IFNG Protein

• Catalog Number: orb257562
• Category: Proteins
• Description: Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 16.8 kDa
• Target: IFN-gamma
• Source: Human IFN-gamma, premium grade (orb257562) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Gln 166 (Accession # AAH70256).
• Expression System: HEK293
• Biological Activity: Immobilized Human IFN-gamma, premium grade at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag with a linear range of 1-10 ng/mL (QC tested).
• Expression Region: Gln 24 - Gln 166
• Endotoxins: 0.1 EU per μg
• NCBI: AAH70256
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Interferon-gamma ,Interferon-γ
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 16.8 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IFN gamma protein

Immobilized Human IFN-gamma, premium grade (Cat. No. orb257562) at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, Fc Tag (Cat. No. orb257559) with a linear range of 1-10 ng/mL (QC tested).