Human IFI16 ELISA Kit

• Catalog Number: orb1173603
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-interferon-inducible protein 16(IFI16). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Gamma-interferon-inducible protein 16(IFI16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-interferon-inducible protein 16(IFI16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Gamma-interferon-inducible protein 16(IFI16) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Tested applications: ELISA
• Reactivity: Human
• Range: 0.16-10 ng/mL
• UniProt ID: Q16666
• Sensitivity: 0.062 ng/mL
• Protein Length: 3.5h
• Alternative names: IFI16, IFNGIP1, Gamma-interferon-inducible protein
• Note: For research use only
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.