Human IA-2 Ab(Insulinoma associated antigen 2 Autoantibody) ELISA Kit

• Catalog Number: orb1736490
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IA-2 Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IA-2 Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IA-2 Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IA-2 Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 62.5-4000 U/mL
• Sensitivity: 27.1 U/mL
• Alternative names: IA2 Ab
• Note: For research use only
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.