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Catalog Number | orb80138 |
---|---|
Category | Proteins |
Description | I-TAC Human Recombinant (Interferon-inducible T-cell alpha chemoattractant) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 8300 Dalton. The I-TAC is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | I TAC |
Solubility (25°C) | It is recommended to reconstitute the lyophilized I-TAC in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be, Phe-Pro-Met-Phe-Lys. |
Biological Origin | E.coli |
Biological Activity | ED50 range=1.0-10 ng/mL, determined by the dose dependent chemotaxis of human lymphocytes cultured in the presence of IL-2. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The CXCL11 was lyophilized from concentrated (1mg/ml) solution in water containing no additives. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human I-TAC protein
> 97% as determined by SDS-PAGE and HPLC. | |
8.3 kDa | |
E.Coli |
Human | |
62.5-4000pg/ml | |
37.5pg/ml |
Human | |
31.25 pg/mL-2000 pg/mL | |
7.8 pg/mL |
Human | |
62.5-4000 pg/mL | |
23 pg/mL |