Human Hypoxia Inducible Factor 1 Alpha (HIF-1α) ELISA Kit

SKU: orb1807474

Description

Human Hypoxia Inducible Factor 1 Alpha (HIF-1α) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenate, Cell lysate
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 62.5-4000pg/mL
• Sensitivity: 25.2 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor

Quick Database Links

Gene Symbol

HIF1a

UniProt

Q16665