Human HLA-B27(Leukocyte Antigen B27) ELISA Kit

• Catalog Number: orb1736484
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-B27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-B27 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 0.32-20 ng/mL
• UniProt ID: O19193
• Sensitivity: 0.12 ng/mL
• Alternative names: HALB27; Human leukocyte antigen B27
• Note: For research use only
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Expiration Date: Please enquire.