Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

SKU: orb1807472

Description

Human High Mobility Group Protein B1 (HMGB-1) ELISA Kit

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 31.25-2000pg/mL
• Sensitivity: 13.7 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

HMGB1, HMG1, HMG3, SBP-1, Amphoterin

Quick Database Links

Gene Symbol

HMGB-1

UniProt

P09429

References

Yun He 1 2, Di Zhang 1, Jiao Liu 1, Junwen Ouyang 1, Chengxiao Zhang 1, Qian Tan 2, Jiaxuan Jiang 1, Kai Hu Ocular Soluble Microneedle Patch Loaded with Immunogenic Cell Death Inducer PKHB1 Peptide for Alleviating Herpes Simplex Keratitis ACS Appl Mater Interfaces, (2025)

PubMed: 40685578

Applications

ELISA

Reactivity

Human