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Catalog Number | orb80136 |
---|---|
Category | Proteins |
Description | HCC-1 Human Recombinant produced in E.coli is a single,non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 8411 Dalton. The HCC-1 is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | HCC 1 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized HCC-1 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN. |
Biological Activity | The Biological activity is calculated by its ability to chemoattract Human monocytes at 5-20 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The CCL14 protein was lyophilized with 20 mM PBS pH-7.4 and 150mM NaCl. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
10.7 kDa | |
Human CCL14, His Tag (orb1184788) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Asn 93 (Accession # Q16627-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
9.71 KDa | |
Mammalian |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95 % by SDS-PAGE and HPLC analyses | |
7.8 kDa |
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