Human Growth Regulated Oncogene Alpha (GROa/CXCL1) ELISA Kit

SKU: orb774889

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3(NAP3).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.8 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CXCL1 ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, GRO-A ELISA Kit, MGSA-A ELISA Kit, NAP3 ELISA Kit, SCYB1 ELISA Kit, Chemokine C-X-C-Motif Ligand 1 ELISA Kit, Melanoma Growth Stimulating Activity Alpha ELISA Kit, Fibroblast Secretory Protein ELISA Kit

Quick Database Links

UniProt

P09341

References

Rui Yuan 1, Qi-Chao Sun 2, Yue-Peng An 1, Qing Zhang 1, Qiong Zhang 1, Yue Li 3 Eriodictyol inhibits the proliferation and inflammatory response in keratinocytes in psoriasis through inactivating DYRK1A-mediated endoplasmic reticulum stress J Asian Nat Prod Res, (2025)

PubMed: 39987551

Applications

ELISA

Reactivity

Human