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Human Growth Regulated Oncogene Alpha (GROa/CXCL1) ELISA Kit
| Catalog Number | orb774889 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3(NAP3). |
| Reactivity | Human |
| Concentration | 1000 pg/mL |
| UniProt ID | P09341 |
| Tested applications | ELISA |
| Application notes | standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 5.8 pg/mL |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | CXCL1 ELISA Kit, GRO1 ELISA Kit, GROa ELISA Kit, G Read more... |
| Note | For research use only |
| Expiration Date | Please enquire. |
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Rui Yuan 1, Qi-Chao Sun 2, Yue-Peng An 1, Qing Zhang 1, Qiong Zhang 1, Yue Li 3 Eriodictyol inhibits the proliferation and inflammatory response in keratinocytes in psoriasis through inactivating DYRK1A-mediated endoplasmic reticulum stress J Asian Nat Prod Res, (2025)
Applications
ELISA
Reactivity
Human
