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Catalog Number | orb257506 |
---|---|
Category | Proteins |
Description | Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 54.3 kDa |
Target | Growth Hormone R |
Source | Human GHR, Fc Tag (orb257506) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912). |
Expression System | HEK293 |
Biological Activity | Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Human GHR, Fc Tag with a linear range of 0.2-3 ng/mL (QC tested). |
Expression Region | Ala 27 - Tyr 264 |
Endotoxins | 1.0 EU per μg |
NCBI | P10912 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | GHR,GHBP,GH receptor Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 54.3 kDa. As a result of glycosylation, the protein migrates as 66-85 kDa under reducing (R) condition, and 135-160 kDa under non-reducing (NR) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human Growth Hormone R protein
Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Human GHR, Fc Tag (Cat. No. orb257506) with a linear range of 0.2-3 ng/mL (QC tested).
Unconjugated | |
95% | |
29.6 kDa | |
Human GHR, His Tag (orb257505) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). |
98.00% | |
The protein has a predicted MW of 28.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
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