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Catalog Number | orb80128 |
---|---|
Category | Proteins |
Description | GRO Alpha Human Recombinant produced in E.coli is a single,non-glycosylated, polypeptide chain containing 73 amino acids and having a molecular mass of 7811 Dalton. The GRO-alpha is purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | GRO a |
Solubility (25°C) | It is recommended to reconstitute the lyophilized CXCL1 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ser-Val-Ala-Thr. |
Biological Origin | E.coli |
Biological Activity | The biological activity was determined by measuring the dose dependent mobilization of intracellular calcium (calcium flux) with human neutrophils. Significant calcium mobilization is observed with 1ng/mL of recombinant human GRO-alpha. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The protein was lyophilized with no additives. |
Alternative names | Growth-regulated protein alpha protein, CXCL1 prot Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human GRO-alpha protein
Greater than 90% as determined by SDS-PAGE. | |
23.9 kDa | |
E.coli |
Greater than 90% as determined by SDS-PAGE. | |
11.9 kDa | |
E.coli |