Human GDNF / ATF / hGDNF Protein, premium grade

• Catalog Number: orb1675340
• Category: Proteins
• Description: Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. The GDNF family members (consists of GDNF, neurturin, artemin and persephin) are structurally similar to transforming growth factor-beta. Unlike other members of the TGF-beta superfamily, which signal through the receptor serine-threonine kinases, GDNF family ligands activate intracellular signalling cascades via the receptor tyrosine kinase Ret. GDNF family ligands binds to GDNF family receptor alpha (GFRalpha) and then the GDNF family ligand-GFR alpha complex binds to and stimulates autophosphorylation of Ret.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 15.1 kDa
• Target: GDNF
• Source: Human GDNF Protein, premium grade (orb1675340) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Ile 211 (Accession # P39905-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human GDNF Protein, premium grade at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag with a linear range of 0.2-4 ng/mL (QC tested).
• Expression Region: Ser 78 - Ile 211
• Endotoxins: 0.1 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 15.1 kDa. The protein migrates as 15-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human GDNF Protein, premium grade (Cat. No. orb1675340) at 2 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag (Cat. No. orb257499) with a linear range of 0.2-4 ng/mL (QC tested).