Human GDF-15 / MIC-1 Protein

• Catalog Number: orb1087553
• Category: Proteins
• Description: Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1). Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS). It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.
• Reactivity: Human
• Tag: N-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 38.8 kDa
• Target: GDF-15
• Source: Human GDF-15, Fc Tag (orb1087553) is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human GFR alpha-like, His Tag at 5 μg/mL (100 μL/well) can bind Human GDF-15, Fc Tag with a linear range of 0.1-6 ng/mL (QC tested).
• Expression Region: Ala 197 - Ile 308
• Endotoxins: 1.0 EU per μg
• NCBI: NP_004855.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 38.8 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 70-90 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

Immobilized Human GFR alpha-like, His Tag (Cat. No. orb545746) at 5 μg/mL (100 μL/well) can bind Human GDF-15, Fc Tag (Cat. No. orb1087553) with a linear range of 0.1-6 ng/mL (QC tested).