Human GAPDH / G3PDH Protein

• Catalog Number: orb257495
• Category: Proteins
• Description: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is also known as Peptidyl-cysteine S-nitrosylase GAPDH, which is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. GAPDH has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. GAPDH can participate in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Also, GAPDH can modulate the organization and assembly of the cytoskeleton. Furthermore, GAPDH is a component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.
• Reactivity: Human
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 36.9 kDa
• Target: GAPDH
• Protein Sequence: AAH01601
• Source: Human GAPDH, His Tag (orb257495) is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).
• Expression System: E. coli
• Expression Region: Gly 2 - Glu 335
• Endotoxins: 1.0 EU per μg
• NCBI: AAH01601
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH7.5
• Alternative names: GAPDH,GAPD,CDABP0047,OK,SW-cl.12
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human GAPDH protein