Human FTO(Alpha-ketoglutaRate-dependent dioxygenase FTO) ELISA Kit

• Catalog Number: orb1146851
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-ketoglutaRate-dependent dioxygenase FTO(FTO) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Range: 0.16-10 ng/mL
• UniProt ID: Q9C0B1
• Sensitivity: 0.059 ng/mL
• Protein Length: 3.5h
• Alternative names: FTO, KIAA1752, fat mass and obesity associated
• Note: For research use only
• Sample Types: serum, plasma and other biological fluids
• Expiration Date: Please enquire.