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Human Flt3-Ligand protein
Catalog Number: orb80029
| Catalog Number | orb80029 |
|---|---|
| Category | Proteins |
| Description | Flt3-Ligand Human Recombinant produced in E.coli is non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17605 Dalton. Flt3-Ligand is purified by proprietary chromatographic techniques. |
| Target | Flt3 Ligand |
| Conjugation | Unconjugated |
| Buffer/Preservatives | The protein was lyophilized with no additives. |
| Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser. |
| Tested applications | SDS-PAGE |
| Biological Origin | E.coli |
| Biological Activity | The ED50 range=0.5-1.0 ng/mL, calculated by the dose-dependant stimulation of the proliferation of human OCMI-AML5 cells. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Flt3-L in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Alternative names | Fms-related tyrosine kinase ligand protein, FLK2 p Read more... |
| Note | For research use only |
SDS-PAGE analysis of Human Flt3-Ligand protein
- Item 1 of 1
Human FLT3L protein (Active) [orb359278]
> 97% as determined by SDS-PAGE and HPLC.
17.6 kDa
E.Coli
100 μg, 500 μg, 10 μg - Item 1 of 3
Human FLT3 Ligand Protein, mFc-His Tag [orb689394]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 45.6 kDa after removal of the signal peptide.
Mammalian
50 μg, 100 μg, 10 μg - Item 1 of 1
Recombinant human FLT3 Ligand protein (Active, HEK293) [orb1817179]
> 95% as determined by SDS-PAGE
24-30 kDa
10 μg, 500 μg, 50 μg 
Human FLT3LG protein [orb391754]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μgFLT3 Ligand Protein, Human, Recombinant (hFc) [orb1960754]
98.00%
44.8 kDa (predicted). Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result.
1 mg, 500 μg, 100 μg
