Human Fibrinogen Mouse Monoclonal Antibody

SKU: orb4238

Description

Human Fibrinogen Mouse Monoclonal Antibody

Research Area

Cardiovascular Research, Metabolism, Thrombosis, Varscular System

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1ug/Test
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 4F7
• Immunogen: Fibrinogen from human plasma
• Target: FGA/FGB/FGG
• Molecular Weight: 340 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FGA; FGA protein; FGB; FGG; Fib2; Fibrin alpha chain; Fibrinogen A alpha polypeptide; Fibrinogen A alpha polypeptide chain; Fibrinogen alpha chain; Fibrinogen B alpha polypeptide; Fibrinogen beta chain; Fibrinogen G alpha polypeptide; Fibrinogen gamma chain; MGC104327; MGC119422; MGC119423; MGC119425; MGC120405; FIBA_HUMAN.

Blank control (black line): HepG2. Primary Antibody (green line): Mouse Anti-human Fibrinogen antibody (orb4238), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-mouse IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

FGA/FGB/FGG

UniProt

P02671