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Catalog Number | orb80027 |
---|---|
Category | Proteins |
Description | FGF19 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids and having a molecular mass of 21.8 kDa.The FGF-19 is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 95.0% as determined by RP-HPLC and SDS-PAGE analysis |
Conjugation | Unconjugated |
Target | FGF 19 |
Solubility (25°C) | Add deionized water to working concentration approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. |
Protein Sequence | MRPLAFSDAG PHVHYGWGDP IRLRHLYTSG PHGLSSCFLR IRADGVVDCA RGQSAHSLLE IKAVALRTVA IKGVHSVRYL CMGADGKMQG LLQYSEEDCA FEEEIRPDGY NVYRSEKHRL PVSLSSAKQR QLYKNRGFLP LSHFLPMLPM VPEEPEDLRG HLESDMFSSP LETDSMDPFG LVTGLEAVRS PSFEK. |
Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of balb/c 3T3 cells is 100-150ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Filtered (0.4 um) and lyophilized from 1mg/ml in 1x PBS, pH 7.4. |
Alternative names | Fibroblast growth factor 19 protein, FGF-19 protei Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
21.8 kDa | |
E.Coli |
Greater than 90% as determined by SDS-PAGE. | |
47.8 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
Unconjugated | |
Greater than 95% as determined by reducing SDS-PAGE. | |
23.5 KDa | |
E. Coli |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 22.3 kDa after removal of the signal peptide. The apparent molecular mass of FGF19-His is approximately 15-25 kDa due to glycosylation. | |
Mammalian |
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