Human Farnesyltransferase Alpha (FNTa) ELISA Kit

SKU: orb778764

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Farnesyltransferase Alpha(FNTa).

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FNTa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FNTa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FNTa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FNTa in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.55 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

FPTA ELISA Kit, PTAR2 ELISA Kit, PGGT1A ELISA Kit, Protein Prenyltransferase Alpha Subunit Repeat Containing 2 ELISA Kit, CAAX farnesyltransferase subunit alpha ELISA Kit, Ras proteins prenyltransferase subunit alpha ELISA Kit

Quick Database Links

UniProt

P49354