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Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

Catalog Number: orb777705

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777705
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Engrailed Homeobox Protein 2(EN2).
Tested applicationsELISA
ReactivityHuman
Range0.32-20 ng/mL
Assay Time3.5h
Concentration20 ng/mL
UniProt IDP19622
Sensitivity0.108 ng/mL
Alternative namesAUTS1 ELISA Kit
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EN2 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typestissue homogenates and other biological fluids
Expiration DatePlease enquire.
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit

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