Human EGF Protein

• Catalog Number: orb762429
• Category: Proteins
• Description: Human epidermal growth factor (EGF) is also known as HOMG4 and URG,and is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Epidermal growth factor can be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. All family members contain one or more repeats of the conserved amino acid sequence. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. Because of the increased risk of cancer by EGF, inhibiting it decreases cancer risk.
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 8.3 kDa
• Target: EGF
• Source: Human EGF Protein, His Tag, premium grade (orb762429) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human EGF Protein, His Tag, premium grade at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag with a linear range of 0.2-5 ng/mL (QC tested).
• Expression Region: Asn 971 - Arg 1023
• Endotoxins: 0.1 EU per μg
• NCBI: NP_001954.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: EGF,URG,HOMG4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

Immobilized Human EGF Protein, His Tag, premium grade (Cat. No. orb762429) at 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. orb257416) with a linear range of 0.2-5 ng/mL (QC tested).