Human Dkk-3 (R335G) Protein

• Catalog Number: orb257976
• Category: Proteins
• Description: Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 37.0 kDa
• Target: Dkk-3
• Protein Sequence: NP_001018067.1 (R335G)
• Source: Human Dkk-3 (R335G), His Tag (orb257976) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Ile 350 (Accession # Q9UBP4-1 (R335G)).
• Expression System: HEK293
• Expression Region: Ala 22 - Ile 350
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001018067.1, R335
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: DKK3,REIC,RIG
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.0 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Dkk-3 protein