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Catalog Number | orb1946838 |
---|---|
Category | Assays and Kits |
Description | Human DHEA-S(Dehydroepiandrosterone Sulfate) ELISA Kit |
Reactivity | Human |
Range | 15.63-1000ng/mL |
Target | DHEA-S |
Sensitivity | 9.38 ng/mL |
Alternative names | Androstenolone sulfate; Prasterone sulfate; Andros Read more... |
Note | For research use only |
Application notes | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human DHEA-S. During the reaction, Human DHEA-S in the sample or standard competes with a fixed amount of Human DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human DHEA-S. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human DHEA-S in tested samples can be calculated by comparing the OD of the samples to the standard curve. |
Sample Types | serum, plasma, Tissue homogenate and Other biological samples |
Expiration Date | Please enquire. |
Human | |
1.563-100ng/ml | |
0.938ng/ml |
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