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Human Complement Factor H (CFH) ELISA Kit

Human Complement Factor H (CFH) ELISA Kit

Catalog Number: orb2667247

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667247
CategoryAssays and Kits
DescriptionHuman Complement Factor H (CFH) ELISA Kit
UniProt IDP08603
Application notesstandard: 10 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFH in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 µg/mL
Sensitivity0.048 µg/mL
Sample Typesserum, plasma and other biological fluids
Alternative namesCF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS
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NoteFor research use only
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    96 T
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    14.063ng/ml

    48 T, 96 T