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Human Complement C4-B (C4B) ELISA Kit

Catalog Number: orb775736

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775736
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement C4-B(C4B).
Tested applicationsELISA
ReactivityHuman
Range4.69-300 ng/mL
Assay Time3.5h
Concentration300 ng/mL
UniProt IDP0C0L5
Sensitivity2.3 ng/mL
Alternative namesCO4 ELISA Kit, CPAMD3 ELISA Kit, Basic complement
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NoteFor research use only
Application notesstandard: 300 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C4B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C4B in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typesserum, plasma and other biological fluids
Expiration DatePlease enquire.
Human Complement C4-B (C4B) ELISA Kit

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