Human Colony Stimulating Factor 2 Receptor Beta (CSF2Rb) ELISA Kit

SKU: orb778165

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2 Receptor Beta(CSF2Rb).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CSF2Rb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CSF2Rb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CSF2Rb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CSF2Rb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.113 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD131 ELISA Kit, CSF2R-B ELISA Kit, IL3-RB ELISA Kit, IL3RB ELISA Kit, CSF2-RB ELISA Kit, Colony Stimulating Factor 2 Receptor,Beta,Low-Affinity(granulocyte-Macrophage) ELISA Kit, GM-CSF/IL-3/IL-5 receptor common beta subunit ELISA Kit

Quick Database Links

UniProt

P32927