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Human Cluster of Differentiation 30 (CD30) ELISA Kit

Catalog Number: orb1949488

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1949488
CategoryAssays and Kits
DescriptionHuman Cluster of Differentiation 30 (CD30) ELISA Kit
ReactivityHuman
Range0.79-50ng/mL
Assay Time3.5H
TargetCD30
UniProt IDP28908
Sensitivity0.47ng/mL
Alternative namesTNFRSF8, CD30, D1S166E, Ki-1, tumor necrosis facto
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CD30. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CD30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CD30, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CD30. You can calculate the concentration of Human CD30 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesSerum, Plasma, Tissue homogenate and Other biological samples
Expiration DatePlease enquire.
Human Cluster of Differentiation 30 (CD30) ELISA Kit