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Human Cell Adhesion Molecule 1 (CADM1) ELISA Kit

SKU: orb777553
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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule 1(CADM1).

Images & Validation

Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CADM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CADM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CADM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CADM1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityHuman
Sample TypesSerum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.072 ng/mL
Concentration10 ng/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
DisclaimerFor research use only

Alternative Names

BL2 ELISA Kit, IGSF4 ELISA Kit, IGSF4A ELISA Kit, NECL2 ELISA Kit, RA175 ELISA Kit, ST17 ELISA Kit, SYNCAM ELISA Kit, TSLC1 ELISA Kit, sTSLC-1 ELISA Kit, synCAM1 ELISA Kit, Tumor Suppressor In Lung Cancer 1 ELISA Kit, Immunoglobulin Superfamily,Member 4 ELISA Kit, Nectin-Like 2 ELISA Kit

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Human Cell Adhesion Molecule 1 (CADM1) ELISA Kit

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Human Cell Adhesion Molecule 1 (CADM1) ELISA Kit (orb777553)

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